Radiant-C Clinical Study
- Radiant-C reduces melanin synthesis by 80%.
- Radiant-C at 0.1% in vitro increases collagen by 50%.
- Radiant-C at 10% in vivo treats acne with 80% of patients (12 people)
- Radiant-C increases collagen synthesis at least twice as much as ascorbic acid.
- Radiant-C inhibits MMP-2 and MMP-9 over 3 times better than ascorbic acid.
- Radiant-C penetrates the skin 4 times better than Magnesium Ascorbyl Phosphate.
- Radiant-C delivers pure Vitamin C 50 times better than ascorbic acid.
- Radiant-C decreases 8-OHdG induced by UV-A.
- Radiant-C decreases p53 expression induced by UV-B.
- Radiant-C protects the cells against UV-B better than other esters of Vitamin C.
COMPARISON OF ABILITY FOR COLLAGEN SYNTHESIS
UPTAKEN CONTENT OF INTRACELLULAR (KERATINOCYTES) ASCORBIC ACID
Tetrahexyldecyl Ascorbate Radiant-C Complex
Increasing Ascorbic acid levels in the cell with the use of Tetrahexyldecyl Ascorbate
In a recent paper presented by Nikko Chemicals of Tokyo Japan and Hiroshima Prefectural University entitled "A new lipophilic L-ascorbic acid derivative '2,3,5,6-0-tetra-2-hexyldecanoyl-L-ascorbiacc id', The synthesis, physical property and the dermatological efficacy", they tested the intercellular absorption of Tetrahexyldecyl Ascorbate as compared to ascorbic acid in epidermis cells. The results showed that Tetrahexyldecyl Ascorbate was significantly more efficient in increasing the levels of ascorbic acid within the epidermis cells. The tests were conducted as follows.
Methods: Epidermis cells (Pam212) were fed with 5, 10,20 uM of Tetrahexyldecyl Ascorbate or 20, 50,200,500 uM of Ascorbic acid, which were non-cytotoxic concentrations, and then left them for 2 hours. After the test cells were harvested by trypsinization, Cell pellets were twice rinsed, and crushed with a potter-type teflon homogenizer. In order to quantify the uptaken concentration of Tetrahexyldecyl Ascorbate as intracellular Ascorbic acid, homogenates were immediately analyzed by HPLC with UV/coulometric ECD method as previously described.
Results: Ascorbic acid or Tetrahexyldecyl Ascorbate of non-cytotoxic concentrations underwent an uptake into Pam212 cells for 2 hours. Quantification of the intracellular Ascorbic acid concentrations were made for each test sample. The concentration of Ascorbic acid found were (1) 12.0 ~M/10c-e~ll for Tetrahexyldecyl Ascorbate and (2) 4.0 pM/10'6 cell for Ascorbic acid (at the same concentration of 20 uM).
Discussion: "Most of the Tetrahexyldecyl Ascorbate administered in the test cells was believed to undergo intercellular absorption as the non-esterolyzed form, because the intact Tetrahexyldecyl Ascorbate (remainder Tetrahexyldecyl Ascorbate) was appreciably detected by HPLC method using coulometric ECD and UV detector. From these results, it was suggested that intracellular Tetrahexyldecyl Ascorbate was to be thoroughly esterolyzed into the Ascorbic acid in the major metabolic pathway, but to be partially esterolyzed into mono-, di-, or tri-ester forms of Ascorbic acid, which was putatively detected at the HPLC retention time between Ascorbic acid and Tetrahexyldecyl Ascorbate in the extracts from Tetrahexyldecyl Ascorbate administered cells). (Probably, Tetrahexyldecyl Ascorbate was slowly esterolyzed into Ascorbic acid through some precursory esters in the cells. While Tetrahexyldecyl Ascorbate is being esterolyzed, one step at a time into the mono-, di, or tri-ester forms, these fonns can reside in the lipids within the cell. It is believed they can provide anti-oxidant activity while maintaining oil solubility)".
The above study suggest that Tetrahexyldecyl Ascorbate can be applied topically in cosmetic applications to improve absorption of Vitamin C (Ascorbic acid) by the skin.