Needle Free Serum Clinical Study
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Biological activity
Production of total collagen, collagens I and III (ex vivo tests)
The action of Needle Free Serum on total collagen production was evaluated on skin explants from a 74 years old donor. Skin explants were either untreated or treated with 0.5% of NF-13 Complex at day 0, day 1, day 4, day 6 and day 8. Explants were then sampled and analyzed at day 0, day 6 and day 11. Total collagen was detected with staining assessed by microscopical observation and image analysis.
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After studying the total collagen, two different types of collagens were quantitatively evaluated by immunostaining: collagen I (in green fluorescent), and collagen III (in dark rose), also known as the youth collagen.
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Results: NF-13 Complex at 0.5% significantly increases collagen I and collagen III synthesis by +179% and +194% respectively after 11 days, versus untreated. Upon aging, the ratio of collagen I / collagen III is increased. With Neodermyl® at 0.5%, this ratio is decreased to the level of a younger skin: it moves from 0.82 to 0.55.
Production of elastin (ex vivo tests)
The action of NF-13 Complex on tropoelastin and elastin production was evaluated by following the same protocol as for collagen I and III. Tropoelastin (in green fluorescent) and elastin (in green fluorescent) were detected by immunostaining assessed by microscopical observation and image analysis.
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Results: NF-13 Complex significantly increases tropoelastin and elastin production by +57% and +190% respectively after 11 days, versus untreated.
Efficacy
Fast improvement of firmness and elasticity (clinical)
The two parameters R0 and R5 were measured by Cutometry to evaluate the performance of NF-13 Complex at 1% on firmness and elasticity.
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Results: Using NF-13 Complex at 1% increases firmness by 13 times and elasticity by 1.6 times in only 15 days. These improvements of skin parameters are sustained after two months of treatment.